Description
BotuGold
For which situation?
- Use: Before and After centrifugation
- Sperm quality: Average to Good, especially for stallions with suspected premature sperm capacity
- Suitable for centrifuging sperm before the freezing procedure
- This diluent is also suitable for sending semen. For refrigerated sperm transport and for centrifugation before freezing, a temperature between 5°C and 15°C should be used
Other Information:
- Available in: 1 sachet for 100ml extender. NB!! 100ml bottle of mineralized water not included!!
- Use: Add 1 sachet to 100ml demineralised water, shake well until powder is dissolved. Wait 30 minutes with the aim of completely dissolving the casein
- Best before date: Extender has a shelf life of 2 years after production date (see imprint sachet)
- Shelf life after mixing: 3 days stored at 2-8°C & 1 year in freezer
- For refrigerated transport, use dilutions of 50 million/ml, keeping at least 2 parts extender to 1 part semen
- If samples are to be centrifuged prior to refrigerated transport, resuspend the spermatozoa at a rate of at least 100×106 sptz/ml
- DO NOT exceed proper cholesterol levels. It is important to adhere to the dilutions in the table below: Sperm Concentration Dilution (v:v)
< 90×106/ml 1:1 Centrifuged Resuspended 100×106sptz/ml
90-120×106/ml 2:1
130-160×106/ml 3:1
170-210×106/ml 4:1
220-250×106/ml 5:1
260-300×106/ml 6:1